Functional assays of gut barrier Integrity and Disease Modelling

  • Functional Assays Of Gut Barrier Integrity and Disease Modelling

Gut Organoid Permeability Assay

Understanding how a drug interacts with the gastrointestinal (GI) tract is critical for predicting its oral bioavailability, safety, and overall clinical success. Traditional 2D cell monolayers and animal models often fail to replicate the structural and functional complexity of the human intestine, limiting their predictive power.

At Greenstone Biosciences, our Gut Organoid harnesses 3D human gut organoids to model the intestinal epithelial barrier with remarkable fidelity. These organoids recapitulate the multicellular architecture, polarity, and absorptive functions of the human intestine, enabling precise evaluation of drug absorption, transport, and potential gut toxicity. 

Key Capabilities

Barrier Function Assessment

Measure integrity and tight junction dynamics to evaluate compound effects on intestinal permeability.

Drug Absorption & Metabolism

Model how compounds cross the intestinal epithelium, informing predictions of oral bioavailability and Metabolism.

Gut Toxicity Insights

Detect off-target effects that compromise epithelial health, reducing risk of GI side effects in patients.

Physiologically Relevant Models

Capture the complexity of the intestinal microenvironment, including nutrient absorption and mucus production.

Why It Matters

Oral delivery remains the most common and patient-preferred route of administration, yet predicting intestinal permeability and tolerability remains a major challenge. Our gut organoid platform provides human-relevant, mechanistic insights that go far beyond traditional in vitro assays. This enables drug developers to:

  • Optimize oral formulations with higher confidence.
  • Predict pharmacokinetics more accurately.
  • Avoid clinical setbacks due to poor absorption or GI intolerance.

From Bench to Clinic

By integrating 3D gut organoid models into early development, the Gut Organoid Permeability Assay bridges a critical gap in translational research. It supports smarter compound selection, reduces reliance on animal models, and improves the likelihood of success in clinical trials.

Shaping the Future of Oral Drug Development

With this platform, Greenstone Biosciences delivers a powerful tool for evaluating oral drug candidates in a clinically relevant context. By closely mimicking the human intestinal epithelium, we help researchers design therapies that are not only effective but also safe, well-tolerated, and optimized for patient use.

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